Global Malaise Trap Program
Large-Scale Sampling for Worldwide Biodiversity Assessments
Develop and standardize protocols for the acquisition and analysis of detailed temporal and spatial information on terrestrial arthropod communities across the globe
Project Outcomes (September 2018)
130 SITES PROCESSED
TOTAL SPECIMENS = 2,082,406
RECORDS WITH BINS = 1,771,353
BINS = 153,988
Complete GMP species accumulation curve for all of the 130 sites completed as of September 2018, indicating progress through 2015 (69 sites completed) and 2017 (117 sites completed).
Variation among sites in observed BIN diversity with latitude (P<0.001 and r2=0.80). The size of points indicates the number of specimens. The P and r2 values reflect overall model statistics while the regression line indicates the significant effect of latitude.
CBG provides a standard sampling kit to all GMP participants. This includes a brand new Townes style Malaise trap and typically a year’s worth of collection bottles. Partners provide ethanol for killing and preserving samples and are responsible for changing the collection bottle once every week for the duration of the flight season.
All collection bottles are shipped for subsequent processing at CBG. Samples are accessioned, specimens were identified to order, arrayed, labeled, databased, and tissue-sampled for genetic analysis. All arthropods are barcoded, with the exception of a few very common species of Collembola, where only a few individuals from each trap sample were analyzed. Standard barcoding protocols are followed to recover the barcode region of cytochrome c oxidase subunit I (COI) gene. The barcode sequences, specimen images and collateral data are uploaded to BOLD under the ‘Global Malaise Program’ campaign. Barcoded specimens are assigned to an existing or new Barcode Index Number (BIN), a proxy for a formal Linnean species name, as outlined by Ratnasingham & Hebert (2013). Identifications are assigned by the BOLD-ID Engine where possible, allowing preliminary species inventories to be completed for each location and facilitating comparisons among them.
Reports are generated upon completion of analysis for a single GMP site. They include a taxonomy list, image library, neighbor-joining tree, and different comparisons with other analyzed GMP locations. The whole program moves forward through collaborative efforts where each country is creating a species inventory and barcode library for itself but shared BINs between countries therefore allow for concurrent identifications between collections.