iBOL 2017

Pre-Conference Workshop Resources

Workshop presentation

    1. Welcome and introduction – Natural History Collection Harvesting Strategies & Logistics (PDF)
    2. Sample Harvesting in Natural History Collections (PDF)
    3. Specimen Digitization (PDF)
    4. Pre-lab Processing (PDF)
    5. Standard Protocols for Natural History Collection Barcoding (PDF)
    6. Data Analysis and Archiving (PDF)
    7. New Developments in the lab (PDF)

Files provided

CCDB and BOLD Templates

Museum Templates

  • Specimen Removal Labels (XLS): Create label place holders for specimen removal from a museum collection. *Need to change the institution on loan to and the plate numbers accordingly.
  • DNA Barcode Labels (XLS): Labels to add to specimens that are tissue sampled on site to mark that they have been sampled for sequencing. *Need to update the sample ID accordingly.
  • Museum Data Entry (XLS): Spreadsheet to enter data while at the museum. The information will also be used by the museum for the loan invoice.

Documentation

Shipping

Other Presentations

Publications

  • Borisenko, A. V., J. E. Sones, and P. D. N. Hebert. 2009. The front-end logistics of DNA barcoding: challenges and prospects. Molecular Ecology Resources 9:27-34.

http://onlinelibrary.wiley.com/doi/10.1111/j.1755-0998.2009.02629.x/abstract

  • Hausmann, A., S. E. Miller, J. D. Holloway, J. R. deWaard, D. Pollock, S. W. J. Prosser and P. D.N. Hebert. 2016. Calibrating the taxonomy of a megadiverse insect family: 3000 DNA barcodes from geometrid type specimens (Lepidoptera, Geometridae). Genome, 2016, 59(9): 671-684.

https://doi.org/10.1139/gen-2015-0197

  • Hebert, P. D. N., J. R. deWaard, E. V. Zakharov, S. W. J. Prosser, J. E. Sones, J. T. A. McKeown, B. Mantle, and J. La Salle. 2013. A DNA ‘Barcode Blitz’: Rapid Digitization and Sequencing of a Natural History Collection. Plos One 8:14.

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0068535

  • Hebert, P. D. N., T. W. A. Braukmann, S. W. J. Prosser, S. Ratnasingham, J. R. deWaard, N. V. Ivanova, D. H. Janzen, W. Hallwachs, S. Naik, J. E. Sones and E. V. Zakharov. 2017. A Sequel to Sanger: Amplicon Sequencing That Scales. BMC Genomics 2018 19:219.

https://doi.org/10.1186/s12864-018-4611-3

  • Hollingsworth, P. M., D. Li, M. van der Bank and A. D. Twyford. 2016. Telling plant species apart with DNA: from barcodes to genome. Phil. Trans. R. Soc. B 371:20150338.

http://dx.doi.org/10.1098/rstb.2015.0338

  • Kuzmina, M. L., T. W. A. Braukmann, A. J. Fazekas, S. W. Graham, S. L. Dewaard, A. Rodrigues, B. A. Bennett, T. A. Dickinson, J. M. Saarela, P. M. Catling, S. G. Newmaster, D. M. Percy, E. Fenneman, A. Lauron-Moreau, B. Ford, L. Gillespie, R. Subramanyam, J. Whitton, L. Jennings, D. Metsger, C. P. Warne, A. Brown, E. Sears, J. R. Dewaard, E. V. Zakharov and P. D. N.  Hebert. 2017. Using Herbarium-Derived DNAs to Assemble a Large-Scale DNA Barcode Library for the Vascular Plants of Canada. Applications in Plant Sciences, 5(12):1700079.

https://doi.org/10.3732/apps.1700079

  • Porco, D., R. Rougerie, L. Deharveng, and P. Hebert. 2010. Coupling non-destructive DNA extraction and voucher retrieval for small soft-bodied Arthropods in a high-throughput context: the example of Collembola. Molecular Ecology Resources 10:942-945.

http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1755-0998.2010.2839.x/abstract

  • Prosser, S. W. J., J. R. deWaard, S. E. Miller, and P. D. N. Hebert. 2016. DNA barcodes from century-old type specimens using next-generation sequencing. Molecular Ecology Resources 16:487-497.

http://onlinelibrary.wiley.com/wol1/doi/10.1111/1755-0998.12474/abstract

Lab resources

http://ccdb.ca/resources/

BOLD resources

http://boldsystems.org/index.php/Resources