Sampling Resources

Experts at the Centre for Biodiversity Genomics at the University of Guelph demonstrate how to prepare museum specimens for DNA Barcoding. 

This video makes use of the documents and protocols available for download below.

Museum Sampling Workflow

A workflow diagram showing the process of sampling, processing, DNA extraction, and doing data analysis and curation for museum specimens.

Files provided

Museum Templates

  • Museum Label Template (XLS): Create place holders labels for specimen removal from a museum collection, as well as DNA barcode labels to be added to specimens after sampling.
  • Museum Harvesting Tracking Sheet (XLS): Spreadsheet to enter auxiliary information (e.g cabinet and taxonomic info) while pulling specimens directly from the Collection.

CCDB and BOLD Templates



Additional Resources

iBOL 2017 Workshop Presentation

Other Presentations

Lab Resources

BOLD Resources


  • Borisenko, A. V., J. E. Sones, and P. D. N. Hebert. 2009. The front-end logistics of DNA barcoding: challenges and prospects. Molecular Ecology Resources 9:27-34.
  • Hausmann, A., S. E. Miller, J. D. Holloway, J. R. deWaard, D. Pollock, S. W. J. Prosser and P. D.N. Hebert. 2016. Calibrating the taxonomy of a megadiverse insect family: 3000 DNA barcodes from geometrid type specimens (Lepidoptera, Geometridae). Genome, 2016, 59(9): 671-684.
  • Hebert, P. D. N., J. R. deWaard, E. V. Zakharov, S. W. J. Prosser, J. E. Sones, J. T. A. McKeown, B. Mantle, and J. La Salle. 2013. A DNA ‘Barcode Blitz’: Rapid Digitization and Sequencing of a Natural History Collection. Plos One 8:14.
  • Hebert, P. D. N., T. W. A. Braukmann, S. W. J. Prosser, S. Ratnasingham, J. R. deWaard, N. V. Ivanova, D. H. Janzen, W. Hallwachs, S. Naik, J. E. Sones and E. V. Zakharov. 2017. A Sequel to Sanger: Amplicon Sequencing That Scales. BMC Genomics 2018 19:219.
  • Hollingsworth, P. M., D. Li, M. van der Bank and A. D. Twyford. 2016. Telling plant species apart with DNA: from barcodes to genome. Phil. Trans. R. Soc. B 371:20150338.
  • Kuzmina, M. L., T. W. A. Braukmann, A. J. Fazekas, S. W. Graham, S. L. Dewaard, A. Rodrigues, B. A. Bennett, T. A. Dickinson, J. M. Saarela, P. M. Catling, S. G. Newmaster, D. M. Percy, E. Fenneman, A. Lauron-Moreau, B. Ford, L. Gillespie, R. Subramanyam, J. Whitton, L. Jennings, D. Metsger, C. P. Warne, A. Brown, E. Sears, J. R. Dewaard, E. V. Zakharov and P. D. N.  Hebert. 2017. Using Herbarium-Derived DNAs to Assemble a Large-Scale DNA Barcode Library for the Vascular Plants of Canada. Applications in Plant Sciences, 5(12):1700079.
  • Porco, D., R. Rougerie, L. Deharveng, and P. Hebert. 2010. Coupling non-destructive DNA extraction and voucher retrieval for small soft-bodied Arthropods in a high-throughput context: the example of Collembola. Molecular Ecology Resources 10:942-945.
  • Prosser, S. W. J., J. R. deWaard, S. E. Miller, and P. D. N. Hebert. 2016. DNA barcodes from century-old type specimens using next-generation sequencing. Molecular Ecology Resources 16:487-497.

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